This video outlines the overview of the urea test The urea slant is comprised of a urea agar, which contains urea and it uses phenol red as the pH indicator and the test is used to determine if the organism is able to break down urea now urea is a waste product from amino acid breakdown so it’s related to a protein catabolic pathway a Negative test which indicates the organism cannot break down urea, will remain the same color the a yellowish-peachish type color is when you start a color change to a pink but not necessarily pink all the way through indicates that there’s a delayed reaction to either urea formation or breakdown in the process and Finally a strong pink color Which is what you’ll often see if the organism is able to digest urea Will indicate that the organism contains the enzyme urease and that enables the organism to break urea down The organism that has a lighter color, also has the enzyme urease But there is a delayed reaction in the breakdown of urea When urea is broken down it’s broken down into ammonia in this pathway And it will cause the pH to rise to above 8.4 so you actually have an alkaline end product in this case. A pH below or an acidic pH a pH below 6.8 will Cause or not cause any color change, so it’ll look like the original starting to color and that is the overview of the urea test